Effect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets

Authors

  • Ahmad Ghorbani Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
  • Nona Zokaei Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
  • Reza Shafiee-Nick Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran
Abstract:

Objective: Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level.This study was undertaken to investigateits direct effect on the islets of Langerhans. Material and Methods: Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer containing 3 or 10 mM glucose in the presence of hydroalcoholic extract of G. Lucidum (1 mg/ml), 3-isobutyl-1-methylxanthine (IBMX, 100 µM) or vehicle. Results: Exposure of islets to the extract increased insulin secretion at basal (3 mM) glucose concentration. Increase of glucose concentration to 10 mM resulted in a significant increase in the rate of insulin secretion. While the IBMX could augment insulin release evoked by 10 mM glucose, the extract failed to modify it. Conclusion: Our results demonstrate that G. lucidum acts directly on the Langerhans islets to increase basal insulin release. 

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Effect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets

OBJECTIVE Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level. This study was undertaken to investigate its direct effect on the islets of Langerhans. MATERIAL AND METHODS Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer contain...

full text

effect of ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets

objective: ganoderma lucidum (g. lucidum) has been suggested to increase serum insulin level.this study was undertaken to investigateits direct effect on the islets of langerhans. material and methods: male albino wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. the islets were incubated for 60 min in krebs bicarbonate buffer containin...

full text

Protective Effect of Capparis spinosa Hydroalcoholic Extract on the Integrity of Rat Pancreatic Islets

Background and Objective: Capparis spinosa L. belongs to the Capparaceae family for which biological roles such as antioxidant, anti-inflammatory, and antidiabetic effects have been reported. Some active chemical groups including flavonoids, phenols, alkaloids, tannins, and minerals have been detected in these plants [Sci Rew1] . This study aimed to extract the C. spinosa alcoholic extract from...

full text

Effect of Ganoderma Lucidum on Cytokine Release by Peritoneal Macrophages

Background: The water-soluble extract of Ganoderma lucidum (Reishi) has been used as an immunomodulator to stimulate spleen cells proliferation and cytokine expression. Objective: To investigate the effect of Ganoderma lucidum (G. lucidum) on cytokine production by mice peritoneal macrophages. Methods: Mice peritoneal macrophages were prepared by intra-peritoneal injection of 5 ml cold PBS. Per...

full text

Effect of Ganoderma lucidum on cytokine release by peritoneal macrophages.

BACKGROUND The water-soluble extract of Ganoderma lucidum (Reishi) has been used as an immunomodulator to stimulate spleen cells proliferation and cytokine expression. OBJECTIVES To investigate the effect of Ganoderma lucidum (G. lucidum) on cytokine production by mice peritoneal macrophages. METHODS Mice peritoneal macrophages were prepared by intra-peritoneal injection of 5 ml cold PBS. P...

full text

Assessment of benzene induced oxidative impairment in rat isolated pancreatic islets and effect on insulin secretion.

Benzene (C6H6) is an organic compound used in petrochemicals and numerous other industries. It is abundantly released to our environment as a chemical pollutant causing widespread human exposure. This study mainly focused on benzene induced toxicity on rat pancreatic islets with respect to oxidative damage, insulin secretion and glucokinase (GK) activity. Benzene was dissolved in corn oil and a...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 2  issue 4

pages  206- 211

publication date 2012-09-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023